In vivo antimycobacterial studies of toussaintine a-chitosan nanocomposites

Authors

Edward Rwegasila, Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, Dar es Salaam, TanzaniaFollow
Joan J.E. Munissi, Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, Dar es Salaam, TanzaniaFollow
Egid B. Mubofu, Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, Dar es Salaam, TanzaniaFollow
Stephen S. Nyandoro, Chemistry Department, College of Natural and Applied Sciences, University of Dar es Salaam, P.O. Box 35061, Dar es Salaam, TanzaniaFollow
Paul Erasto, National Institute for Medical Research (NIMR), P.O. Box 9653, Dar es Salaam, Tanzania *Correspondence: joan.munissi@udsm.ac.tzFollow

Section

Biological Sciences

Abstract

Chitosan (CS, molecular weight (MW) 20.2 kDA, stability of 210 °C and degree of deacetylation (DD) 73.31%) was obtained by deacetylation of chitin extracted from shrimp (Litopenaeus vannamei) shell wastes. The encapsulation of the studied bioactive natural product, toussaintine A (TA) isolated from the leaves of Toussaintia orientalis, on a chitosan-tripolyphosphate (CS/TPP) nanoformulation was attained through ionotropic gelation. Characterization of pure CS, CS/TPP and TA-CS/TPP nanocomposites was carried out by FTIR and SEM. The encapsulation efficiency and loading capacity of the TA were 69.33 and 0.46%, respectively. The in vitro release kinetics established an initial release of 27% of TA in the initial six hours followed by a slow and maintained release up to 72 h. The in vivo antimycobacterial acitivities of both TA and TA-CS/TPP nanocomposites against Mycobacterium indicus pranii (MIP) employing Galleria mellonella larvae as an infection model were evaluated. TA-CS/TPP nanocomposite formulations exhibited remarkable effectiveness against MIP than free TA.

Recommended Citation

Rwegasila, Edward; Munissi, Joan J.E.; Mubofu, Egid B.; Nyandoro, Stephen S.; and Erasto, Paul (2018) "In vivo antimycobacterial studies of toussaintine a-chitosan nanocomposites," Tanzania Journal of Science: Vol. 44: Iss. 2, Article 2.
Available at:https://doi.org/10.65085/2507-7961.1746